Inhibition of FGF receptor activity in retinal ganglion cell axons causes errors in target recognition

S. McFarlane, E. Cornel, E. Amaya and C. E. Holt

Neuron 17:245-254 (1996) (Full Article, 524K)

Abstract

Native fibroblast growth factor receptor (FGFR) function was inhibited in developing Xenopus

retinal ganglion cells (RGCs) by in vivo transfection of a dominant negative FGFR. Axons

expressing the dominant negative protein advanced at 60% of the normal speed, but nevertheless

navigated appropriately in the embryonic optic pathway. When they neared the optic tectum,

however, many axons made erroneous turns, causing them to bypass rather than enter their target.

By contrast, RGC axons expressing nonfunctional FGFR mutants entered the tectum correctly.

These findings demonstrate a role for FGFR signaling in the extension and targeting of RGC

axons and suggest that receptor tyrosine kinase/growth factor interactions play a critical function

in establishing initial connectivity in the vertebrate visual system.

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