A method for generating transgenic frog embryos

E. Amaya and K.L. Kroll

Methods in Molecular Biology, Molecular Embryology: Methods and Protocols. Edited by Paul Sharpe and Ivor Mason. Humana Press Inc., Totowa, NJ. (1999) 97:393-414.

Abstract

The early amphibian embryo has been widely used as a model organism for studying early

vertebrate development. In this chapter we describe in detail a new and very efficient method

for generating transgenic Xenopus embryos. At the end of the chapter we also introduce a new

method for fertilizing in vitro matured oocytes.

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