Name: pUAST

Conformation: linear

Overhang: five_prime, three_prime, blunt

Minimum Site Length: 5 bases

Maximum Number of Cuts: all

Included: all commercial, prototypes only

Noncutters: AgeI, AloI, AscI, AvrII, BaeI, BbvCI, BstEII, FseI, FspAI, MluI, NaeI, NheI, PI-PspI, PI-SceI, PacI, PmeI, PshAI, PsrI, RsrII, SacII, SanDI, SapI, SfiI, SgfI, SgrAI, SpeI, SrfI, SwaI, Tth111I

Name Sequence Site Length Overhang Frequency Cut Positions
Eco47III AGCGCT 6 blunt 1 961
EcoRV GATATC 6 blunt 1 3748
NruI TCGCGA 6 blunt 1 3258
PsiI TTATAA 6 blunt 1 629
SmaI CCCGGG 6 blunt 1 2918
SnaBI TACGTA 6 blunt 1 2824
StuI AGGCCT 6 blunt 1 759
AflII CTTAAG 6 five_prime 1 1344
BglII AGATCT 6 five_prime 1 13
Bpu10I CCTNAGC 6 five_prime 1 8396
Bsp1407I TGTACA 6 five_prime 1 4619
ClaI ATCGAT 6 five_prime 1 3503
Eco31I GGTCTC 6 five_prime 1 6622
EcoNI CCTNNNNNAGG 6 five_prime 1 5168
EcoRI GAATTC 6 five_prime 1 1
MfeI CAATTG 6 five_prime 1 596
NarI GGCGCC 6 five_prime 1 1961
NcoI CCATGG 6 five_prime 1 2309
NdeI CATATG 6 five_prime 1 7732
NotI GCGGCCGC 8 five_prime 1 20
PpuMI RGGWCCY 7 five_prime 1 4991
XbaI TCTAGA 6 five_prime 1 40
XhoI CTCGAG 6 five_prime 1 28
AatII GACGTC 6 three_prime 1 7483
BstXI CCANNNNNNTGG 6 three_prime 1 2950
Eam1105I GACNNNNNGTC 6 three_prime 1 6561
KpnI GGTACC 6 three_prime 1 38
SacI GAGCTC 6 three_prime 1 2636
SphI GCATGC 6 three_prime 1 8522
Sse8387I CCTGCAGG 8 three_prime 1 8528
BsaBI GATNNNNATC 6 blunt 2 508, 3378
HpaI GTTAAC 6 blunt 2 9, 609
OliI CACNNNNGTG 6 blunt 2 3363, 3718
PvuII CAGCTG 6 blunt 2 1912, 8748
AclI AACGTT 6 five_prime 2 6787, 7160
AsuII TTCGAA 6 five_prime 2 4280, 8026
BamHI GGATCC 6 five_prime 2 745, 8506
BclI TGATCA 6 five_prime 2 866, 1516
BsePI GCGCGC 6 five_prime 2 4417, 7887
BseYI CCCAGC 6 five_prime 2 2689, 5972
BspLU11I ACATGT 6 five_prime 2 1330, 3262
DraII RGGNCCY 6 five_prime 2 4991, 7537
SalI GTCGAC 6 five_prime 2 2701, 3560
SexAI ACCWGGT 7 five_prime 2 908, 2999
AlwNI CAGNNNCTG 6 three_prime 2 2562, 6084
ApaI GGGCCC 6 three_prime 2 4116, 8196
BglI GCCNNNNNGGC 6 three_prime 2 2476, 6681
DrdI GACNNNNNNGTC 6 three_prime 2 5776, 7645
FalI AAGNNNNNCTT 6 three_prime 2 3063, 3095
PstI CTGCAG 6 three_prime 2 8528, 8771
PvuI CGATCG 6 three_prime 2 2155, 6931
XcmI CCANNNNNNNNNTGG 6 three_prime 2 2523, 2947
BtrI CACGTC 6 blunt 3 3939, 5617, 8711
PmaCI CACGTG 6 blunt 3 3980, 5288, 5576
AarI CACCTGC 7 five_prime 3 4344, 5161, 7960
AflIII ACRYGT 6 five_prime 3 1330, 3262, 8708
Cfr10I RCCGGY 6 five_prime 3 1957, 3801, 6641
HindIII AAGCTT 6 five_prime 3 5451, 8488, 8512
BciVI GTATCC 6 three_prime 3 1635, 5871, 7398
DraIII CACNNNGTG 6 three_prime 3 1138, 3980, 5579
PflMI CCANNNNNTGG 6 three_prime 3 198, 2211, 2310
XmnI GAANNNNTTC 6 blunt 4 3504, 4902, 4952, 7160
AcyI GRCGYC 6 five_prime 4 1961, 2216, 7098, 7480
ApaLI GTGCAC 6 five_prime 4 5422, 5982, 7228, 7725
AvaI CYCGRG 6 five_prime 4 28, 2916, 3846, 5706
BspHI TCATGA 6 five_prime 4 1513, 6388, 7396, 7501
BspMI ACCTGC 6 five_prime 4 4344, 5161, 7960, 8517
BplI GAGNNNNNCTC 6 three_prime 4 22, 54, 2503, 2535
BseRI GAGGAG 6 three_prime 4 304, 1144, 3053, 3759
BsrDI GCAATG 6 three_prime 4 1253, 1706, 6622, 6796
MmeI TCCRAC 6 three_prime 4 216, 5883, 6067, 8511
TaqII GACCGA 6 three_prime 4 6909, 6935, 7068, 7094
BalI TGGCCA 6 blunt 5 2204, 2939, 3724, 4060, 8248
PfoI TCCNGGA 6 five_prime 5 2182, 2372, 4004, 5547, 7594
BfiI ACTGGG 6 three_prime 5 1788, 2782, 3980, 5577, 6601
Eco57I CTGAAG 6 three_prime 5 343, 1054, 2584, 6216, 7228
GsuI CTGGAG 6 three_prime 5 2984, 4027, 5288, 5531, 6631
NspI RCATGY 6 three_prime 5 1334, 3266, 7589, 8404, 8522
ScaI AGTACT 6 blunt 6 7041, 8537, 8556, 8575, 8594, 8613
Esp3I CGTCTC 6 five_prime 6 1004, 4003, 4592, 5549, 7551, 7593
FauI CCCGC 5 five_prime 6 2362, 3665, 4491, 7655, 7665, 7815
Ksp632I CTCTTC 6 five_prime 6 321, 3405, 4430, 7356, 7875, 8866
BsmI GAATGC 6 three_prime 6 233, 597, 690, 1534, 2142, 2464
BtsI GCAGTG 6 three_prime 6 225, 447, 685, 5184, 6961, 6981
BsaAI YACGTR 6 blunt 7 2824, 3980, 4050, 5288, 5357, 5506, 5576
HindII GTYRAC 6 blunt 7 9, 609, 1918, 2703, 3562, 3734, 7102
SspI AATATT 6 blunt 7 477, 3280, 4611, 4686, 5335, 7365, 8431
VspI ATTAAT 6 five_prime 7 1273, 3282, 3476, 4901, 5294, 6733, 8433
BsgI GTGCAG 6 three_prime 7 430, 825, 3670, 3945, 5195, 5221, 5613
HaeII RGCGCY 6 three_prime 7 963, 1964, 3709, 3787, 5916, 8645, 8665
TspGWI ACGGA 5 three_prime 7 3400, 3886, 5672, 7001, 7343, 8395, 8688
AccI GTMKAC 6 five_prime 8 2702, 3561, 3863, 4297, 5440, 5691, 8009, 8670
AvaII GGWCC 5 five_prime 8 2037, 3022, 3796, 4991, 5469, 6699, 6921, 8276
StyI CCWWGG 6 five_prime 8 346, 891, 2219, 2309, 4520, 5230, 5242, 7784
BcgI CGANNNNNNTGC 6 three_prime 8 2879, 2913, 4133, 4167, 7066, 7100, 8143, 8177
MslI CAYNNNNRTG 6 blunt 9 2413, 3363, 3718, 4811, 5199, 6813, 6972, 7331, 8405
SmlI CTYRAG 6 five_prime 9 28, 1344, 1979, 3050, 4959, 5774, 6036, 6313, 7181
TfiI GAWTC 5 five_prime 9 164, 188, 3177, 3500, 3505, 4549, 5191, 7756, 8791
BsrBI CCGCTC 6 blunt 10 1727, 1824, 4486, 7402, 7822, 8550, 8569, 8588, 8607, 8626
PleI GAGTC 5 five_prime 10 291, 388, 1802, 2524, 2869, 5147, 6047, 6550, 8366, 8625
TatI WGTACW 6 five_prime 10 1327, 4619, 5061, 7039, 7715, 8535, 8554, 8573, 8592, 8611
Eco57MI CTGRAG 6 three_prime 10 343, 1054, 2584, 2984, 4027, 5288, 5531, 6216, 6631, 7228
PpiI GAACNNNNNCTC 7 three_prime 10 3048, 3080, 3835, 3867, 5694, 5726, 6351, 6383, 7193, 7225
BseMII CTCAG 5 three_prime 11 271, 2072, 2400, 3539, 5957, 6366, 6532, 7049, 7710, 8335, 8353
ApoI RAATTY 6 five_prime 12 1, 132, 660, 877, 2947, 3147, 3242, 4948, 5213, 5325, 5382, 5429
CfrI YGGCCR 6 five_prime 12 20, 1825, 2093, 2202, 2282, 2657, 2835, 2937, 3722, 4058, 6949, 8246
XhoII RGATCY 6 five_prime 12 13, 46, 745, 1696, 2645, 6309, 6320, 6406, 6418, 7186, 7203, 8506
BseSI GKGCMC 6 three_prime 12 2420, 2686, 2788, 2847, 3985, 4116, 5426, 5575, 5986, 7232, 7729, 8196
BsmAI GTCTC 5 five_prime 13 1004, 4003, 4480, 4592, 5549, 6622, 7398, 7551, 7593, 7824, 8284, 8362, 8623
Tsp45I GTSAC 5 five_prime 13 77, 1095, 1449, 1715, 3574, 3711, 4712, 4754, 5004, 5307, 6817, 7028, 7604
EciI GGCGGA 6 three_prime 13 1054, 1788, 2459, 2480, 2828, 3788, 3949, 4014, 5544, 5609, 5730, 5876, 6704
HgaI GACGC 5 five_prime 14 1003, 1044, 2224, 2446, 2538, 4020, 4127, 4933, 5531, 5779, 6357, 7087, 7645, 8176
Hpy99I CGWCG 5 three_prime 15 2554, 2704, 3972, 4121, 4518, 4927, 5468, 5589, 5773, 6567, 6830, 7795, 8192, 8285, 8715
BccI CCATC 5 five_prime 16 278, 1129, 1305, 1896, 2094, 2187, 2649, 3308, 3330, 3399, 3774, 4188, 6605, 6729, 7016, 8119
EcoRII CCWGG 5 five_prime 16 908, 1749, 1853, 1964, 2233, 2284, 2303, 2372, 2555, 2999, 3035, 3701, 4004, 5547, 5815, 5828
BsaXI ACNNNNNCTCC 6 three_prime 16 207, 237, 2846, 2876, 2918, 2948, 2966, 2996, 4472, 4502, 4516, 4546, 7765, 7795, 7809, 7839
FokI GGATG 5 five_prime 17 1914, 2152, 2166, 2199, 2269, 2331, 2581, 3988, 4271, 4745, 5564, 6527, 6708, 6995, 7638, 8033, 8280
SfaNI GCATC 5 five_prime 18 662, 1317, 1591, 2174, 2177, 2216, 2309, 3279, 4773, 4795, 5137, 5765, 6817, 7008, 7257, 7616, 7710, 8505
HphI GGTGA 5 three_prime 20 1210, 1727, 1837, 2000, 2379, 2439, 3106, 3513, 4427, 4494, 5219, 6404, 6631, 7047, 7253, 7288, 7572, 7581, 7815, 7882
TauI GCSGC 5 three_prime 20 22, 25, 1011, 1397, 1730, 1827, 1909, 2069, 2160, 2496, 3833, 4030, 4041, 5518, 5529, 5870, 6954, 7076, 7305, 7701
TspDTI ATGAA 5 three_prime 20 223, 604, 1527, 2596, 2995, 3236, 3290, 3390, 3513, 4417, 4569, 4963, 4999, 5328, 5476, 6452, 6529, 6832, 7893, 8274
TspRI CASTG 5 three_prime 21 225, 454, 692, 849, 2795, 2967, 3112, 3632, 3638, 4769, 4893, 5075, 5191, 5431, 6076, 6089, 6360, 6509, 6614, 6961, 6988
Hin4I GAYNNNNNVTC 6 three_prime 22 488, 520, 912, 944, 1688, 1720, 2592, 2624, 2841, 2846, 2873, 2878, 3639, 3671, 4516, 4548, 6547, 6579, 6621, 6653, 7765, 7797
MboII GAAGA 5 three_prime 22 273, 338, 351, 2577, 2609, 3281, 3386, 3392, 3630, 3684, 3828, 4304, 4417, 5729, 6330, 6401, 7156, 7234, 7343, 7892, 8005, 8883
SduI GDGCHC 6 three_prime 22 1483, 1898, 2420, 2516, 2636, 2686, 2788, 2847, 3051, 3853, 3985, 4088, 4116, 5426, 5575, 5707, 5986, 7147, 7232, 7729, 8196, 8224
BsrI ACTGG 5 three_prime 24 849, 1794, 2531, 2788, 2967, 3105, 3535, 3638, 3859, 3986, 4762, 4893, 5075, 5305, 5572, 5699, 6076, 6089, 6201, 6607, 6725, 6768, 7037, 7207
BbvI GCAGC 5 five_prime 25 431, 634, 998, 1298, 1727, 1921, 2355, 2980, 3518, 3671, 3946, 4896, 4899, 5188, 5222, 5331, 5606, 5997, 6087, 6090, 6296, 6599, 6990, 7601, 8757
TseI GCWGC 5 five_prime 25 444, 622, 1011, 1286, 1740, 1909, 2343, 2968, 3531, 3684, 3959, 4909, 4912, 5176, 5235, 5319, 5594, 6010, 6075, 6078, 6284, 6612, 6978, 7589, 8745