Cover competition images 2020
1) Kyungtae Lim: Human fetal lung organoids, Leica SP8 inverted microscope, 20X; Surfactant Protein C(pink), HT2-280(green), SOX2(white), Nuclei(blue)
2) Tessa Hughes: Airway Branching The image is of an embryonic mouse lung, taken on a Leica SP8 confocal at 40x magnification. Some basic analysis in Fiji. The white staining is E-cadherin, indicating the epithelium (airways at this stage), and all the other colours are from the confetti mouse strain, all progeny will have the same colour as the parent cell, allowing us to trace stem cell lineages.
3) Joseph Jose Thottacherry: Adult Drosophila Gut expressing histone RFP marking nucleus of all the cells, Spinning disk confocal microscope, analyzed using Fiji (ImageJ), with a 40x air objective. The movie shows the gut undergoing peristalsis.
4) Joseph Jose Thottacherry: Adult Drosophila Gut expressing Histone-RFP and Arm-GFP imaged using Spinning disk with 60x objective and analyzed using Fiji(imageJ) software. False coloring shows the nucleus of all cells (His-RFP) in Cyan and Armadillo labelling in Magenta in the stem cells (tiny nucleus in the stem cells can be seen inside magenta positive cells).
5) Jonathan Gadsby: Gentle Slopes - Colour coded Z-stack of HK2 (Human Kidney 2) cells immunolabelled for PI(4,5)P2 and DAPI. 26 x 0.2 um Z stack taken on the Zeiss Airyscan microscope using the 63x objective in Airyscan Fast mode (processed using Airyscan Strength 6 in ZEN). The PI(4,5)P2 stack has been colour coded according to Z height using the Fire LUT in FIJI (Image J), and this was merged with a maximal projection of the DAPI channel (white) in Adobe Photoshop.
6) Andrea Frapporti: Resolution Shift A single nucleus from a 200-cell stage C. elegans embryo immunostained for the histone modification H3K9me2, a hallmark of silent chromatin, and imaged via confocal and STED microscopy. The image has been composed so as to highlight the transition from the diffraction-limited confocal capture to the super-resolved STED acquisition, that allows for a net and visible gain in structural information.
7) Oriol Llorà Batlle: Drosophila melanogaster salivary glands, captured with an SP8 confocal microscope. Cyan shows the nuclei and magenta the actin staining with Phalloidin. It was captures by Oriol Llora Batlle form the Brand Lab.
