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27.01.20 Gurdon lab reveal the interaction between immune response and regeneration in tadpoles

last modified Feb 05, 2020 10:17 AM
Can Aztekin of the Gurdon lab and colleagues identify the cells of the immune response that are involved in creating the conditions for regeneration in the tadpole
27.01.20 Gurdon lab reveal the interaction between immune response and regeneration in tadpoles

The regeneration response in the tadpole tail

The myeloid lineage is required for the emergence of a regeneration permissive environment following Xenopus tail amputation

Aztekin C, Hiscock TW, Butler R, De Jesús Andino F, Robert J, Gurdon JB, Jullien J. (2020) Development. pii: dev.185496. DOI: 10.1242/dev.185496.

 

Abstract from the paper

Regeneration-competent vertebrates are considered to suppress inflammation faster than non-regenerating ones. Hence, understanding the cellular mechanisms affected by immune cells and inflammation can help develop strategies to promote tissue repair and regeneration.

Here, we took advantage of naturally occurring tail regeneration-competent and -incompetent developmental stages of Xenopus tadpoles. We first establish the essential role of the myeloid lineage for tail regeneration in the regeneration-competent tadpoles. We then reveal that upon tail amputation there is a reduction in amputation-induced apoptosis levels in the myeloid lineage, which in turn promotes tissue remodelling, and ultimately leads to the relocalization of the regeneration-organizing cells responsible for progenitor proliferation. These cellular mechanisms failed to be executed in regeneration-incompetent tadpoles.

We demonstrate that regeneration incompetency is characterized by inflammatory myeloid cells whereas regeneration competency is associated with reparative myeloid cells. Moreover, treatment of regeneration-incompetent tadpoles with immune-suppressing drugs restores myeloid lineage-controlled cellular mechanisms.

Collectively, our work reveals the effects of differential activation of the myeloid lineage on the creation of a regeneration-permissive environment and could be further exploited to devise strategies for regenerative medicine purposes.

Aztekin schematic regeneration

 

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Read the earlier paper from this research project about Regeneration Organizing Cells.

Read more about research in the Gurdon lab.

Watch John Gurdon describe his research on YouTube.

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

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