skip to primary navigationskip to content

31.10.18 Cell polarisation of fly midgut epithelium provides better model for mammalian epithelia

last modified Nov 16, 2018 01:09 PM
The St Johnston lab's discovery of the mechanism for epithelial cell polarisation in the fly gut disproves the idea that the same conserved system establishes apical-basal polarity in all epithelia

Access the recommendation on F1000Prime 

An alternative mode of epithelial polarity in the Drosophila midgut

Chen J et al. (2018) PLoS Biology 16. DOI: 10.1371/journal.pbio.3000041.

Author summary 

The Drosophila midgut is lined by a single-layered epithelium that acts as a barrier to the environment while allowing for nutrient uptake and related physiological processes. To fulfill these roles, midgut epithelial cells are highly polarised, with a pronounced asymmetric distribution of cellular components.Previous work in Drosophila revealed a conserved set of factors governing cell polarity, and it is thought that this network of proteins underlies all examples of polarity in Drosophila (and other organisms).

Here, we demonstrate that the epithelial cells in the Drosophila midgut are not polarised by these canonical polarity factors but instead rely on the integrin adhesion complex. Thus, there are two types of epithelia in Drosophila that polarise using fundamentally different mechanisms.

Chen alternative polarity

A section of the midgut epithelium stained for F-actin (red) and DNA (blue) containing an aPKC null mutant clone marked by GFP expression (green). The mutant cells polarise normally and show no obvious phenotype. 

Abstract from the paper

Apical–basal polarity is essential for the formation and function of epithelial tissues, whereas loss of polarity is a hallmark of tumours. Studies in Drosophila have identified conserved polarity factors that define the apical (Crumbs, Stardust, Par-6, atypical protein kinase C [aPKC]), junctional (Bazooka [Baz]/Par-3), and basolateral (Scribbled [Scrib], Discs large [Dlg], Lethal [2] giant larvae [Lgl]) domains of epithelial cells. Because these conserved factors mark equivalent domains in diverse types of vertebrate and invertebrate epithelia, it is generally assumed that this system underlies polarity in all epithelia.

Here, we show that this is not the case, as none of these canonical factors are required for the polarisation of the endodermal epithelium of the Drosophila adult midgut. Furthermore, like vertebrate epithelia but not other Drosophila epithelia, the midgut epithelium forms occluding junctions above adherens junctions (AJs) and requires the integrin adhesion complex for polarity.

Thus, Drosophila contains two types of epithelia that polarise by fundamentally different mechanisms. This diversity of epithelial types may reflect their different developmental origins, junctional arrangement, or whether they polarise in an apical–basal direction or vice versa. Since knock-outs of canonical polarity factors in vertebrates often have little or no effect on epithelial polarity and the Drosophila midgut shares several common features with vertebrate epithelia, this diversity of polarity mechanisms is likely to be conserved in other animals.


Read more about research in the St Johnston lab.

Watch Daniel St Johnston talk about his research in this short video.

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

combinedLogo x3 trans2018


Share this

Liver organoids: from basic research to therapeutic applications

NSUN2 introduces 5-methylcytosines in mammalian mitochondrial tRNAs

The roles of DNA, RNA and histone methylation in ageing and cancer

Separating Golgi proteins from cis to trans reveals underlying properties of cisternal localization

Sequencing cell-type-specific transcriptomes with SLAM-ITseq

Mature sperm small-RNA profile in the sparrow: implications for transgenerational effects of age on fitness

Single-cell transcriptome analyses reveal novel targets modulating cardiac neovascularization by resident endothelial cells following myocardial infarction

Derivation and maintenance of mouse haploid embryonic stem cells

Establishment of porcine and human expanded potential stem cells

Adapting machine-learning algorithms to design gene circuits

Lgr5+ stem/progenitor cells reside at the apex of a heterogeneous embryonic hepatoblast pool

Identification of a regeneration-organizing cell in the Xenopus tail

Citrullination of HP1γ chromodomain affects association with chromatin

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

A transmissible RNA pathway in honey bees

METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation

A Secreted RNA Binding Protein Forms RNA-Stabilizing Granules in the Honeybee Royal Jelly

The Human Lung Cell Atlas - A high-resolution reference map of the human lung in health and disease

A Compendium of Mutational Signatures of Environmental Agents

Characteristics and homogeneity of N6-methylation in human genomes

Link to full list on PubMed