skip to primary navigationskip to content
 

10.07.18 Chromatin accessibility is a central factor in successful reprogramming in oocytes

last modified Jul 18, 2018 11:10 PM
In this Cell Reports article the Gurdon and Livesey labs show an association between the local chromatin state and whether genes are activated by or resistant to reprogramming
10.07.18 Chromatin accessibility is a central factor in successful reprogramming in oocytes

Fig.2F extract: Signal associated with open chromatin at TSSs of reprogrammed genes.

Chromatin Accessibility Impacts Transcriptional Reprogramming in Oocytes

Miyamoto K et al. (2018) Cell Reports 24: 304-311. DOI: 10.1016/j.celrep.2018.06.030

 

Highlights from the paper

  • ATAC-seq reveals chromatin accessibility changes during reprogramming in oocytes
  • Genes with open promoters are preferentially activated during reprogramming
  • Transcription factors play a role in transcriptional reprogramming in oocytes
  • Closed chromatin is associated with reprogramming-resistant genes

 

Summary from the paper

Oocytes have a remarkable ability to reactivate silenced genes in somatic cells. However, it is not clear how the chromatin architecture of somatic cells affects this transcriptional reprogramming. Here, we investigated the relationship between the chromatin opening and transcriptional activation.

We reveal changes in chromatin accessibility and their relevance to transcriptional reprogramming after transplantation of somatic nuclei into Xenopus oocytes. Genes that are silenced, but have pre-existing open transcription start sites in donor cells, are prone to be activated after nuclear transfer, suggesting that the chromatin signature of somatic nuclei influences transcriptional reprogramming. There are also activated genes associated with new open chromatin sites, and transcription factors in oocytes play an important role in transcriptional reprogramming from such genes.

Finally, we show that genes resistant to reprogramming are associated with closed chromatin configurations. We conclude that chromatin accessibility is a central factor for successful transcriptional reprogramming in oocytes.

++++++++++++++

 

Watch John Gurdon describe his research in this short video.

Read more about research in the Gurdon lab and the Livesey lab.

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

combinedLogo x3 trans2018

 

Share this

A Secreted RNA Binding Protein Forms RNA-Stabilizing Granules in the Honeybee Royal Jelly

The Human Lung Cell Atlas - A high-resolution reference map of the human lung in health and disease

A Compendium of Mutational Signatures of Environmental Agents

Characteristics and homogeneity of N6-methylation in human genomes

Comparative Epigenomics Reveals that RNA Polymerase II Pausing and Chromatin Domain Organization Control Nematode piRNA Biogenesis

Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis

Dorsal-ventral differences in neural stem cell quiescence are induced by p57KIP2/Dacapo

Crypt fusion as a homeostatic mechanism in the human colon

TaDa! Analysing cell type-specific chromatin in vivo with Targeted DamID

A single-cell molecular map of mouse gastrulation and early organogenesis

Theory of mechanochemical patterning in biphasic biological tissues

Identification of functional long non-coding RNAs in C. elegans

The proneural wave in the Drosophila optic lobe is driven by an excitable reaction-diffusion mechanism

A walk through tau therapeutic strategies

Labeling strategies matter for super-resolution microscopy: a comparison between HaloTags and SNAP-tags

Stem Cell-Derived Human Gametes: The Public Engagement Imperative

Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment

Comparative Epigenomics Reveals that RNA Polymerase II Pausing and Chromatin Domain Organization Control Nematode piRNA Biogenesis

Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis

Link to full list on PubMed