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11.01.19 Role for RNA-binding protein IMP in the fine control of Drosophila egg chamber development

last modified Jan 14, 2019 02:51 PM
The St Johnston lab uncover a novel regulatory step in the Notch signalling pathway in follicle cells, whereby localisation of Kuzbanian to the apical domain promotes Notch cleavage, inducing cell differentiation
11.01.19 Role for RNA-binding protein IMP in the fine control of Drosophila egg chamber development

Fig S1 (excerpt): Wild-type egg chambers expressing IMP-GFP (green), stained for actin (red) and DNA (blue).

Drosophila IMP regulates Kuzbanian to control the timing of Notch signalling in the follicle cells

Weronika Fic, Celia Faria, Daniel St Johnston (11.01.2019) Development,  dev.168963. DOI: 10.1242/dev.168963

 

Abstract from the paper

The timing of Drosophila egg chamber development is controlled by a germline Delta signal that activates Notch in the follicle cells to induce them to cease proliferation and differentiate.

Here we report that follicle cells lacking the RNA-binding protein IMP go through one extra division due to a delay in the Delta-dependent S2 cleavage of Notch. The timing of Notch activation has previously been shown to be controlled by cis-inhibition by Delta in the follicle cells, which is relieved when the miRNA pathway represses Delta expression. imp mutants are epistatic to Delta mutants and give an additive phenotype with belle and dicer mutants, indicating that IMP functions independently of both cis-inhibition and the miRNA pathway.

We find that the imp phenotype is rescued by over-expression of Kuzbanian, the metalloprotease that mediates the Notch S2 cleavage. Furthermore, Kuzbanian is not enriched at the apical membrane in imp mutants, accumulating instead in late endosomes.

Thus, IMP regulates Notch signalling by controlling the localisation of Kuzbanian to the apical domain, where Notch cleavage occurs, revealing a novel regulatory step in the Notch pathway.

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Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

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