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21.06.18 RNA helicase DDX3X is indirect regulator of breast cancer cell cycle

last modified Aug 16, 2018 02:19 PM
The Kouzarides lab identify how DDX3X plays oncogenic role in breast cancer cells via effect on expression and downstream function of KLF4
21.06.18 RNA helicase DDX3X is indirect regulator of breast cancer cell cycle

Model of DDX3X-dependent regulation of cell cycle through repression of KLF4 expression.

DDX3X RNA helicase affects breast cancer cell cycle progression by regulating expression of KLF4

Cannizzaro E et al. (2018) FEBS Lett  592: 2308–2322. DOI: 10.1002/1873-3468.13106

 

Abstract from the paper

DDX3X is a multifunctional RNA helicase with documented roles in different cancer types. Here, we demonstrate that DDX3X plays an oncogenic role in breast cancer cells by modulating the cell cycle.

Depletion of DDX3X in MCF7 cells slows cell proliferation by inducing a G1 phase arrest. Notably, DDX3X inhibits expression of Kruppel-like factor 4 (KLF4), a transcription factor and cell cycle repressor. Moreover, DDX3X directly interacts with KLF4 mRNA and regulates its splicing. We show that DDX3X-mediated repression of KLF4 promotes expression of S-phase inducing genes in MCF7 breast cancer cells.

These findings provide evidence for a novel function of DDX3X in regulating expression and downstream functions of KLF4, a master negative regulator of the cell cycle.

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Read more about research in the Kouzarides lab.

Watch Tony Kouzarides describe his research and additional 'matchmaking' and fundraising activities on video.

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

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