New technique allows identification of recessive suppressor mutations in mammalian cells
Genome-wide genetic screening with chemically mutagenised haploid embryonic stem cells
Forment, JV et al. (2017) Nature Chemical Biology 13, 12–14. doi: 10.1038/nchembio.2226
[Published online 31 October 2016]
A PDF file can be accessed here.
Abstract from paper
In model organisms, classical genetic screening via random mutagenesis provides key insights into the molecular bases of genetic interactions, helping defining synthetic-lethality, synthetic-viability and drug-resistance mechanisms. The limited genetic tractability of diploid mammalian cells, however, precludes this approach. Here, we demonstrate the feasibility of classical genetic screening in mammalian systems by using haploid cells, chemical mutagenesis and next-generation sequencing, providing a new tool to explore mammalian genetic interactions.
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