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31.10.16 Jackson group demonstrate how haploid ES cells can provide new genetic screening platform

last modified Jan 26, 2017 02:51 PM
This Nature Chemical Biology publication from Steve Jackson's group reveals a genetic screening platform based on haploid embryonic stem cells
31.10.16 Jackson group demonstrate how haploid ES cells can provide new genetic screening platform

New technique allows identification of recessive suppressor mutations in mammalian cells

Genome-wide genetic screening with chemically mutagenised haploid embryonic stem cells

Forment, JV et al. (2017) Nature Chemical Biology 13, 12–14. doi: 10.1038/nchembio.2226

[Published online 31 October 2016] 

A PDF file can be accessed here. 

 

Abstract from paper

In model organisms, classical genetic screening via random mutagenesis provides key insights into the molecular bases of genetic interactions, helping defining synthetic-lethality, synthetic-viability and drug-resistance mechanisms. The limited genetic tractability of diploid mammalian cells, however, precludes this approach. Here, we demonstrate the feasibility of classical genetic screening in mammalian systems by using haploid cells, chemical mutagenesis and next-generation sequencing, providing a new tool to explore mammalian genetic interactions.

 

Read more about research in the Jackson lab.

Watch Steve Jackson describe his research on video.

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

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