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18.12.17 Novel method for detecting cell-type-specific lncRNA-genome interactions

last modified Dec 19, 2017 10:27 PM
In this Nature Structural and Molecular Biology article, Cheetham and Brand demonstrate an effective technique to examine the role of lncRNA-chromatin interactions
18.12.17 Novel method for detecting cell-type-specific lncRNA-genome interactions

Schematic representation of RNA-DamID (excerpt from Fig. 1).

RNA-DamID reveals cell-type-specific binding of roX RNAs at chromatin-entry sites

Cheetham SW and Brand AH (2017) Nature Structural & Molecular Biology 

DOI:10.1038/s41594-017-0006-4 (Advance online publication).

Abstract

Thousands of long noncoding RNAs (lncRNAs) have been identified in eukaryotic genomes, many of which are expressed in spatially and temporally restricted patterns. Nonetheless, the roles of the majority of these transcripts are still unknown. One of the mechanisms by which lncRNAs function is through the modulation of chromatin states. To assess the functions of lncRNAs, we developed RNA-DamID, a novel approach that detects lncRNA–genome interactions in a cell-type-specific manner in vivo with high sensitivity and accuracy. Identifying the cell-type-specific genome occupancy of lncRNAs is vital to understanding their mechanisms of action in development and disease. We used RNA-DamID to investigate targeting of the lncRNAs in the Drosophila dosage-compensation complex (DCC) and show that initial targeting is cell-type specific.

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Read more about research in the Brand lab.

Watch Andrea Brand describe her research in this video

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

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