skip to primary navigationskip to content
 

08.08.2017 Buffering role for miR-7 in developing optic lobe

last modified Aug 09, 2017 09:01 AM
In this Cell Reports paper, Caygill and Brand present evidence that the transformation of neuroepithelial cells into neural stem cells in the developing optic lobe of the brain is buffered against environmental stress by the microRNA miR-7.

miR-7 buffers differentiation in the developing Drosophila visual system

Elizabeth E. Caygill and Andrea H. Brand (2017) Cell Reports 20, 1255–1261.

DOI: 10.1016/j.celrep.2017.07.047

 

Author’s summary

Many microRNAs have been discovered in different tissues during development but their roles are often unclear. Here we show that the microRNA miR-7 has a specific role in buffering stem cell production in the Drosophila brain under conditions of environmental stress. In the optic lobe, the visual processing centre of the brain, the formation of neural stem cells (neuroblasts) and their progeny (neurons) needs to match that in the eye to coordinate the synaptic connections made between the two organs. We demonstrate that miR-7 enables the timely production of neural stem cells and buffers their generation against environmental stress. 

 

Summary from the paper

The 40,000 neurons of the medulla, the largest visual processing center of the Drosophila brain, derive from a sheet of neuroepithelial cells. During larval development a wave of differentiation sweeps across the neuroepithelium converting neuroepithelial cells into neuroblasts that sequentially express transcription factors specifying different neuronal cell fates. The switch from neuroepithelial cells to neuroblasts is controlled by a complex gene regulatory network and marked by the expression of the proneural gene l’sc. We discovered that microRNA miR-7 is expressed at the transition between neuroepithelial cells and neuroblasts. We showed that miR-7 promotes neuroepithelial cell to neuroblast transition by targeting downstream Notch effectors to limit Notch signaling. miR-7 acts as a buffer to ensure a precise and stereotypical pattern of transition is maintained even under conditions of environmental stress, echoing the role miR-7 plays in the eye imaginal disc. This common mechanism reflects the importance of robust visual system development.

 

Read more about research in the Brand lab.

Watch Andrea Brand describe her work in this short video.

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

combinedLogo x3 trans2018

 

Share this

Immune Cell Dynamics Unfolded by Single-Cell Technologies

Chromatin Accessibility Impacts Transcriptional Reprogramming in Oocytes

Integrin α2 marks a niche of trophoblast progenitor cells in first trimester human placenta

Inhibition of the acetyltransferase NAT10 normalizes progeric and aging cells by rebalancing the Transportin-1 nuclear import pathway

SLAM-ITseq: Sequencing cell type-specific transcriptomes without cell sorting

SRSF3 maintains transcriptome integrity in oocytes by regulation of alternative splicing and transposable elements

scmap: projection of single-cell RNA-seq data across data sets

Single-cell transcriptomics reveals a new dynamical function of transcription factors during embryonic hematopoiesis

Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48

The developmental origin of brain tumours: a cellular and molecular framework

Bioinformatics challenges and perspectives when studying the effect of epigenetic modifications on alternative splicing

ATM orchestrates the DNA-damage response to counter toxic non-homologous end-joining at broken replication forks

Extracellular Forms of Aβ and Tau from iPSC Models of Alzheimer's Disease Disrupt Synaptic Plasticity

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Predominant Asymmetrical Stem Cell Fate Outcome Limits the Rate of Niche Succession in Human Colonic Crypts

G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst

Link to full list on PubMed