skip to primary navigationskip to content
 

18.08.18 Resolving the cellular basis of mouse pancreas development

last modified Aug 09, 2018 10:37 AM
The Simons and Huch labs, with Cambridge Stem Cell Institute colleagues, tease out the cellular mechanisms by which the pancreas develops its branched ductal structures
18.08.18 Resolving the cellular basis of mouse pancreas development

Clones of labelled stem cells in developing mouse pancreas

Defining Lineage Potential and Fate Behavior of Precursors during Pancreas Development

Sznurkowska MK et al. (2018) Dev Cell Jul 18. pii: S1534-5807(18)30553-7. DOI: 10.1016/j.devcel.2018.06.028. [Epub ahead of print]

Abstract from the paper

Pancreas development involves a coordinated process in which an early phase of cell segregation is followed by a longer phase of lineage restriction, expansion, and tissue remodeling. By combining clonal tracing and whole-mount reconstruction with proliferation kinetics and single-cell transcriptional profiling, we define the functional basis of pancreas morphogenesis.

We show that the large-scale organization of mouse pancreas can be traced to the activity of self-renewing precursors positioned at the termini of growing ducts, which act collectively to drive serial rounds of stochastic ductal bifurcation balanced by termination. During this phase of branching morphogenesis, multipotent precursors become progressively fate-restricted, giving rise to self-renewing acinar-committed precursors that are conveyed with growing ducts, as well as ductal progenitors that expand the trailing ducts and give rise to delaminating endocrine cells.

These findings define quantitatively how the functional behavior and lineage progression of precursor pools determine the large-scale patterning of pancreatic sub-compartments.

+++++++

Read more about research in the Simons lab.

Watch Ben Simons describe his research on video.

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

combinedLogo x3 trans2018

 

Share this

Mature sperm small-RNA profile in the sparrow: implications for transgenerational effects of age on fitness

Single-cell transcriptome analyses reveal novel targets modulating cardiac neovascularization by resident endothelial cells following myocardial infarction

Derivation and maintenance of mouse haploid embryonic stem cells

Establishment of porcine and human expanded potential stem cells

Adapting machine-learning algorithms to design gene circuits

Lgr5+ stem/progenitor cells reside at the apex of a heterogeneous embryonic hepatoblast pool

Identification of a regeneration-organizing cell in the Xenopus tail

Citrullination of HP1γ chromodomain affects association with chromatin

A critical but divergent role of PRDM14 in human primordial germ cell fate revealed by inducible degrons

A transmissible RNA pathway in honey bees

METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation

A Secreted RNA Binding Protein Forms RNA-Stabilizing Granules in the Honeybee Royal Jelly

The Human Lung Cell Atlas - A high-resolution reference map of the human lung in health and disease

A Compendium of Mutational Signatures of Environmental Agents

Characteristics and homogeneity of N6-methylation in human genomes

Comparative Epigenomics Reveals that RNA Polymerase II Pausing and Chromatin Domain Organization Control Nematode piRNA Biogenesis

Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis

Dorsal-ventral differences in neural stem cell quiescence are induced by p57KIP2/Dacapo

Crypt fusion as a homeostatic mechanism in the human colon

Link to full list on PubMed