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16.05.19 Scientists find new type of cell that helps tadpoles’ tails regenerate

last modified May 16, 2019 07:47 PM
16.05.19 Scientists find new type of cell that helps tadpoles’ tails regenerate

Regeneration-organizing cells outline the advancing edge of a regenerating tadpole tail

Identification of a regeneration- organizing cell in the Xenopus tail

Aztekin C et al. (2019) Science 364 (6441):653-658. DOI: 10.1126/science.aav9996


Press release from the Wellcome Trust/ Cancer Research UK Gurdon Institute

Researchers at the University of Cambridge have uncovered a specialised population of skin cells that coordinate tail regeneration in frog tadpoles. These ‘Regeneration-Organizing Cells’ help to explain one of the great mysteries of nature and may offer clues about how this ability might be achieved in mammalian tissues.

It has long been known that some animals can regrow their tails following amputation – Aristotle observed this in the fourth century B.C. – but the mechanisms that support such regenerative potential remain poorly understood. 

Using ‘single-cell genomics’, scientists at the Wellcome Trust/ Cancer Research UK Gurdon Institute at the University of Cambridge developed an ingenious strategy to uncover what happens in different tadpole cells when they regenerate their tails. 

Recent Cambridge-led advances in next-generation sequencing mean that scientists can now track which genes are turned on (being expressed) throughout a whole organism or tissue, at the resolution of individual cells. This technique, known as ‘single-cell genomics’, makes it possible to distinguish between cell types in more detail based on their characteristic selection of active genes. 

These breakthroughs are beginning to reveal a map of cellular identities and lineages, as well as the factors involved in controlling how cells choose between alternative pathways during embryo development to produce the range of cell types in adults. 

Using this technology, Can Aztekin and Dr Tom Hiscock – under the direction of Dr Jerome Jullien – made a detailed analysis of cell types involved in regeneration after damage in African Clawed Frog tadpoles (Xenopus laevis). Details are published today in the journal Science.

Dr Tom Hiscock says: “Tadpoles can regenerate their tails throughout their life; but there is a two-day period at a precise stage in development where they lose this ability. We exploited this natural phenomenon to compare the cell types present in tadpoles capable of regeneration and those no longer capable.” 

The researchers found that the regenerative response of stem cells is orchestrated by a single sub-population of epidermal (skin) cells, which they termed Regeneration-Organizing Cells, or ROCs. 

Can Aztekin says: “It’s an astonishing process to watch unfold. After tail amputation, ROCs migrate from the body to the wound and secrete a cocktail of growth factors that coordinate the response of tissue precursor cells. These cells then work together to regenerate a tail of the right size, pattern and cell composition.”

In mammals, many tissues such as the skin epidermis, the intestinal epithelium and the blood system, undergo constant turnover through life. Cell lost through exhaustion or damage are replenished by stem cells. However, these specialised cells are usually dedicated to tissue sub-lineages, while the ability to regenerate whole organs and tissues has been lost in all but a minority of tissues such as liver and skin.

Professor Benjamin Simons, a co-author of the study says: “Understanding the mechanisms that enable some animals to regenerate whole organs represents a first step in understanding whether a similar phenomenon could be reawakened and harnessed in mammalian tissues, with implications for clinical applications.”


C.A. is funded by University of Cambridge and Cambridge Trust.  T.W.H., J.C.M., B.D.S.,  J.J. and J.B.G. are funded by the Wellcome Trust.  T.W.H. is also supported by EMBO; B.D.S. by the Royal Society; and J.C.M. by EMBL and Cancer Research UK.



Read more about research in the Gurdon and Simons labs

Watch John Gurdon and Ben Simons describe their work in these short YouTube videos.

Studying development to understand disease

The Gurdon Institute is funded by Wellcome and Cancer Research UK to study the biology of development, and how normal growth and maintenance go wrong in cancer and other diseases.

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