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Meritxell Huch

huchMeri Huch PhD, Wellcome Trust Sir Henry Dale Fellow.

Europe PMC | Pubmed

Winner of the 2013 NC3Rs Award

Wellcome-Beit Prize Fellow 2014

University of Cambridge film 'Fighting cancer: animal research at Cambridge' featuring Meri Huch


Stem cells and tissue regeneration in liver and pancreas

2016 HuchHow can we repair diseased liver and pancreas? In adult mammals, many tissues have the capacity to self-renew to maintain healthy function and after damage. But the capacity for cell turnover varies. In the intestine and stomach, adult stem cell populations are constantly replenishing, while in the liver and pancreas cell proliferation is limited.

Chronic liver disease and pancreatic cancer are strongly associated with inflammation and tissue damage, which activates stem cells and progenitor cells to repair lost tissue.

Our goal is to understand the activation mechanism in order to harness it for therapeutic strategies. We have established a novel culture system for liver organoids, which allows the massive and infinite expansion of mouse liver cells into three-dimensional structures that resemble functional liver tissue. When transplanted into a mouse model of liver disease (‘FAH –/–’), these cells partially rescued the liver phenotype. We also work with pancreas cells and diseased human liver cells in culture, and are testing how well our models can represent in vivo pathology.

Selected publications:

• Human primary liver cancer-derived organoid cultures for disease modeling and drug screening. Broutier L, Mastrogiovanni G, Verstegen MM, Francies HE, Gavarró LM, Bradshaw CR, Allen GE, Arnes-Benito R, Sidorova O, Gaspersz MP, Georgakopoulos N, Koo BK, Dietmann S, Davies SE, Praseedom RK, Lieshout R, IJzermans JNM, Wigmore SJ, Saeb-Parsy K, Garnett MJ, van der Laan LJ, Huch M. Nature Medicine (2017) Dec;23(12):1424-1435. doi: 10.1038/nm.4438. Epub 2017 Nov 13. 29131160

• The hope and the hype of organoid research. Huch M, Knoblich JA, Lutolf MP, Martinez-Arias A. Development. (2017) Mar 15;144(6):938-941. PMID: 28292837

• Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. Broutier L, Andersson-Rolf A, Hindley CJ, Boj SF, Clevers H, Koo BK, Huch M. Nature Protocols (2016) Sep; 11(9): 1724-43. PMID: 27560176

• Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver. Huch M, Gehart H, van Boxtel R, Hamer K, Blokzijl F, Verstegen MM, Ellis E, van Wenum M, Fuchs SA, de Ligt J, van de Wetering M, Sasaki N, Boers SJ, Kemperman H, de Jonge J, Ijzermans JN, Nieuwenhuis EE, Hoekstra R, Strom S, Vries RR, van der Laan LJ, Cuppen E, Clevers H. Cell (2015) Jan 15;160(1-2):299-312. PMID: 25533785

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Huch M, Dorrell C, Boj SF, van Es JH, Li VSW, van de Wetering M, Sato T, Hamer K, Sasaki N, Finegold MJ, Haft A, Vries R, Grompe M, Clevers H.  Nature (2013) Feb 14;494: 247-50. PMID: 23354049


Video: Meet Meri Huch

Plain English

The liver is a very slowly dividing organ but, when damaged by toxins present on the food or other environmental agents, it starts the machinery to regenerate itself and bring the organ back to its original resting state. Our aim is to understand the molecular mechanism by which liver or other adult cells sense the damage infringed to the tissue and start the repairing process. Understanding these mechanisms is crucial to improve our knowledge on the basics of cancer initiation, as during tumorigenesis, similar mechanisms have to be put in place to activate the resting cells to start proliferating. 

We are also interested in developing tools to study human physiology and disease in vitro in a petri dish. Because of their numerous biomedical implications in studies of hepatitis, drug testing, as well as transplantation for chronic liver diseases, scientists around the world have unsuccessfully attempted for decades to regenerate primary liver cells. 

We have recently achieved that challenge by establishing a novel liver culture system named 'liver organoid culture' that allows the massive and infinite expansion of mouse liver cells into 3D structures that resemble functional liver tissue in a dish. Of note, when these cells are grown in vitro and transplanted into a mouse model of an inherited liver disease, which resembles the Tyrosinemia type I human liver disease, the cultured and transplanted liver cells continued to grow in the recipient livers and alleviated the pathology of the disease by prolonging the survival of the transplanted mice, thus proving their therapeutic potential.


Luigi Aloia • Robert Arnes • Laura Broutier • Lucia Cordero Espinoza • Nikitas Georgakopoulos • Mewanthi Flaminia Kaluthantrige Don • Patricia Inacio • Gianmarco Mastrogiovanni • Mikel McKie • Kathy Oswald • Nicole Prior