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Jenny Gallop

2017 Gallop2014Jenny Gallop PhD, Wellcome Research Career Development Fellow, ERC Independent Starting Researcher, Member of the Department of Biochemistry

Gallop Group websiteEurope PMC | Pubmed

 

 

 

Membranes, actin and morphogenesis

How do cells generate and maintain their characteristic shapes? The cell membrane, as the boundary of the cell, is moulded into shape by dynamic remodelling of its links to the actin cytoskeleton during cell division, polarisation, movement, differentiation and for everyday housekeeping.

In disease, the actin machinery is hijacked by invading pathogens. Some actin regulators are overexpressed and redeployed during cancer metastasis, and control of the actin cytoskeleton can be disrupted in genetic diseases, causing intellectual disability, kidney dysfunction and other problems.

We are studying how actin filaments polymerise at two types of specialised structures at the cell membrane: filopodia, which are fingerlike protrusions, and endocytic vesicles, which bud inwards to bring in components from the membrane or environment.

We have developed model systems using phospholipid bilayers and frog egg extracts that allow us to follow the molecular events of actin self-assembly in different contexts. By focusing on unusual predictions from these in vitro assays, we work out how the actin cytoskeleton is regulated by imaging cells in accessible, native developmental contexts in fruit fly and frog embryos.

Selected publications:

• Richier B*, Inoue Y*, Dobramysl U, Friedlander J, Brown NH, Gallop JL. (2018) Integrin signaling downregulates filopodia during muscle-tendon attachment. J Cell Sci. Aug 16;131(16).

• Daste F*, Walrant A*, Holst MR*, Gadsby JR*, Mason J, Lee JE, Brook D, Mettlen M, Larsson E, Lee SF, Lundmark R, Gallop JL. (2017) Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature. J Cell Biol. 216: 3745-3765.

• Urbančič V, Butler R, Richier B, Peter M, Mason J, Livesey FJ, Holt CE, Gallop JL. (2017) Filopodyan: An open-source pipeline for the analysis of filopodia. J Cell Biol. 216: 3405-3422.

• Gallop JL, Walrant A, Cantley LC, Kirschner MW. (2013) Phosphoinositides and membrane curvature switch the mode of actin polymerization via selective recruitment of toca-1 and Snx9. Proc Natl Acad Sci 110: 7193-7198

• Lee K*, Gallop JL*, Rambani K, Kirschner MW. (2010) Self-assembly of filopodia-like structures on supported lipid bilayers. Science, 329:1341-1345.

• McMahon HT, Gallop JL. (2005) Membrane curvature and mechanisms of dynamic cell membrane remodelling. Nature. 438: 590-596.

*joint first authors

Gallop Group 2017

Video: Meet Jenny Gallop

Co-workers

Ulrich Dobramysl • Jonathan Gadsby • Pantelis Savvas Ioannou • Bishara Marzook • Julia Mason • Kathy Oswald