Azim Surani

Director of germline and epigenetics research

Research summary

The human germline

We study primordial germ cells (PGCs), precursors to eggs and sperm, in the early embryo. We have established principles of early human development with a focus on human PGC (hPGC) specification. A unique epigenetic resetting follows in the germline after hPGC specification. Our work shows that SOX17 is the key regulator of human, but not mouse, germ cell fate. By developing in vitro models, and with authentic hPGCs from human embryos, we have also established how pluripotent stem cells gain competence for germ cell and somatic fates in human.

Expand summary

These findings are important for studies on human pluripotent stem cells and regenerative medicine. The inheritance of genetic and epigenetic information from the germline through the totipotent state affects human development and disease for generations.

Whereas SOX17–BLIMP1 apparently initiate the epigenetic programme in early human germline, BLIMP1–PRDM14 play a similar role in mouse germline, resulting in the comprehensive erasure of DNA methylation (except for some resistant loci), X-reactivation and imprints erasure, followed by re-establishment of sperm- and oocyte-specific imprints. Defects in these gamete-specific imprints lead to a variety of human disease syndromes.

We have also examined mitochondrial DNA (mtDNA) in PGCs, showing evidence for selection against mitochondria that harbour mutations. This mechanism is imperfect and can account for inherited mtDNA disorders.

Diagram of primordial germ cell development

What we know about primordial germ cell development: Primordial germ cells are the first cell type to be specified in vivo in early human development. We have developed a protocol using specific signalling molecules to generate primordial germ cell-like cells in vitro from human pluripotent stem cells. We found that SOX17 is a critical determinant of human germ cell fate.

Azim Surani colour portrait

Selected publications

  • Kobayashi T et al. (2021) Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells. Cell Reports 37(2):109812. DOI: 10.1016/j.celrep.2021.109812.

    October 12, 2021

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  • Sybirna A et al. (2020) A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons. Nat Commun 11: 1282. DOI: 10.1038/s41467-020-15042-0.

    March 9, 2020

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  • Hackett JA et al. (2018) Tracing the transitions from pluripotency to germ cell fate with CRISPR screening. Nat Commun 9(1): 4292. DOI: 10.1038/s41467-018-06230-0.

    October 16, 2018

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  • Floros VI et al. (2018) Segregation of mitochondrial DNA heteroplasmy through a developmental genetic bottleneck in human embryos. Nat Cell Biol 20: 144– 151. DOI: 10.1038/s41556-017-0017-8.

    January 15, 2018

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  • Kobayashi T et al. (2017) Principles of early human development and germ cell program from conserved model systems. Nature 546: 416–420. DOI: 10.1038/nature22812.

    June 7, 2017

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  • Murakami K et al. (2016) NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. Nature 529: 403–407. DOI: 10.1038/nature16480.

    January 11, 2016

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  • Tang WWC et al. (2015) A Unique Gene Regulatory Network Resets the Human Germline Epigenome for Development. Cell 161(6): 1453–1467. DOI: 10.1016/j.cell.2015.04.053.

    June 4, 2015

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  • Irie N et al. (2015) SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate. Cell 160: 253–268. DOI: 10.1016/j.cell.2014.12.013.

    January 15, 2015

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Biography

Prof Azim Surani PhD CBE FRS FMedSci
Director of Germline and Epigenetics Research, Wellcome Senior Investigator, Member of the University Department of Physiology, Development and Neuroscience

The discovery of genomic imprinting in 1984 by Surani was important for advances in mammalian development and the field of epigenetics.  Mammalian genomes show epigenetic symmetry in totipotent zygotes because the chromosomes retain a memory of their parental origin with heritable DNA methylation tags, which regulate monoallelic expression of parental alleles of ‘imprinted’ genes. These genes have a role in mammalian development, growth, and behaviour; aberrant imprints underlie some human diseases.

Notably, Surani also elucidated the genetic basis for mammalian primordial germ cell specification in mice and humans, which initiates the unique mammalian germline epigenetic program, including erasure and reestablishment of parental imprints. His continuing research is on early human development, the origin of germ cells during gastrulation, and epigenetic inheritance.

Surani was born in Kenya and received a PhD at Cambridge University under Sir Robert Edwards (Nobel laureate, 2010) in 1975, and was elected as Marshall-Walton Professor in 1992, and then as Director of Germline and Epigenetics Research in 2013 at the Gurdon Institute.

He is a Fellow of the Royal Society and the Academy of Medical Sciences. His awards include the William Bate Hardy Prize, a Royal Medal for mammalian development, Rosenstiel Award for epigenetic regulation of gene expression in mammalian embryos, ISSCR McEwen Award for Innovation, and the Canada Gairdner International Award for genomic imprinting and epigenetics.

Notable achievements and honours

  • 2022
    Mendel Medal, Genetics Society
  • 2018
    Canada Gairdner International Award
  • 2017
    Linacre Lecture (St John’s College, Cambridge)
  • 2014
    McEwen Award for Innovation, International Society for Stem Cell Research
  • 2014
    Jawaharlal Nehru Fellowship, Government of India
  • 2010
    Galton Lecture, The Galton Institute
  • 2010
    Royal Medal, Royal Society
  • 2010
    Mendel Lecture (Brno, Czech Republic)
  • 2007
    Commander of the British Empire for services to biology
  • 2007
    Lewis S Rosenstiel Award for Distinguished Work in Basic Medical Science
  • 2004
    Sir Dorabji Tata Distinguished Professor, NCBS Institute (Bangalore) and Distinguished Fellow, Nehru Center
  • 2001
    Fellow of the Academy of Medical Sciences
  • 1999
    Wellcome-Burroughs Research Pioneer Award, National Institute of Child Health and Human Development (USA)
  • 1994
    Doctor of Science honoris causa, University of Uppsala (Sweden)
  • 1994
    Member of Academia Europaea
  • 1993
    Member of European Molecular Biology Organisation
  • 1992
    Associate Fellow of the Third World Academy of Sciences
  • 1986 & 1992
    BBSRC Individual Merit Award
  • 1991
    William Bate Hardy Prize, Cambridge Philosophical Society
  • 1990
    Menten Visiting Professor of Pathology, University of Pittsburgh (USA)
  • 1990
    Fellow of the Royal Society

Research group

  • Lynn Froggett

    Lab Administrator

  • Dr Theresa Gross-Thebing

    Visiting Scholar

  • Dr Wolfram Gruhn

    Research Associate

  • Dr Mei Gu

    Research Assistant/ Lab Manager

  • Dr Naoko Irie

    Senior Research Associate

  • Dr Geraldine Jowett

    Research Associate

  • Dr Jitesh Neupane

    Research Associate

  • Dr So Shimamoto

    Visiting Scholar